mini–IVF with own oocytes and partner’s sperm includes COS cycle, Ivf or ICSI, embryo cultivation, and embryo transfer, and costs 4,295.00 EUR per cycle.
IVF or in vitro fertilization is an advanced assisted reproduction technique that involves controlled ovarian stimulation, oocyte retrieval, sperm collection and sperm preparation, and embryo generation in the IVF laboratory. As soon as the embryos are generated, all of them are transferred to the Time—Lapse incubator for further gestation. The embryo development is closely monitored by the embryology Pros via intelligent apps that not only stream the video content in real–time but also depict and record the photo and video content showing every moment of the embryo gestation. IVF is an exclusive embryo generation technique designed for the couples facing extreme male factor infertility or both the male and female infertility issues. Designed for all the couples, every IVF cycle is tailored for every couple according to the couple’s needs. If it is impossible to do IVF with own oocytes and sperm, the fertility Pros may recommend the couple to undergo IVF with egg donation, IVF with sperm donation, IVF with egg and sperm donation (fresh cycle), or IVF with embryo donation (fresh or frozen cycle). Mini–IVF or mild IVF is an exclusive treatment bundle with the minimal stimulation treatment protocol.
There are four main IVF bundles’ types classified according to the ovarian stimulation medication dosage:
●natural IVF (IVF without medicated ovarian stimulation)
●ultra–mini IVF (IVF with an ultra–light dosage of ovarian stimulation medication)
●mini IVF (IVF with a minimal dosage of ovarian stimulation medication)
●IVF (IVF with a moderate or high dosage of ovarian stimulation medication)
Four main fertilization techniques used by Fertility Pros for the embryo generation event are:
●conventional fertilization
●ICSI
●IMSI
●PICSI
Selecting the IVF bundle according to the number of trials:
●one IVF–ICSI/IMSI/PICSI trail
●two IVF–ICSI/IMSI/PICSI trails
●three IVF–ICSI/IMSI/PICSI trails
●multi–trailed IVF–ICSI/IMSI/PICSI
Decoding the abbreviations tagged with IVF:
●conventional fertilization (oocyte and sperm are placed in one Petri dish without any further micromanipulations. The sperm cells must be labeled as ones with ‘High potency’: powerful enough to perform the natural penetration of the oocyte’s layers (external layer) and the middle layer and to ‘introduce’ the intended father’s genome’s content to the intended mother’s genome content).
●ICSI (intracytoplasmic sperm injection involves placing and fixing the oocyte in the IVF workstation using the oocyte–holding micro–pipette and introducing the spermatozoon caught by its tail and immobilized by the sperm injection micropipette into the oocyte cytoplasm manually. The sperm injection pipettes are designed with long and sharp tips that gently penetrate (puncture) the oocyte without disruption of its structure).
●IMSI (intracytoplasmic morphologically selected sperm injection involves the manual penetration of the mature oocyte with the sperm injecting micro–pipette but the spermatozoon introduced in the oocyte’s cytoplasm is featured with the label ‘top quality sperm cell.’ IMSI technique was designed for boosting the chances to generate the embryo with an ideal content by introducing only the top–quality sperm cell in the oocyte’s cytoplasm).
●PIСSI (physiological intracytoplasmic sperm injection involves the selection of the best–quality sperm for fertilization before injection into the oocyte. Sperm cells are placed into a special PICSI dish with the samples of hyaluronan hydrogel. Hyaluronan hydrogel is a natural biopolymer that ‘envelopes’ the oocyte. The sperm cells that bound to the hyaluronan are immobilized and isolated by the fertility Pro for further usage in ICSI. the sperm’s quality doubles down).
What happens after the sperm cell penetrated the oocyte?
The blended genome contents generate the embryo with a whole new chromosomal profile as the oocyte is a haploid cell with 23 chromosomes, and the spermatozoon is also a haploid cell with 23 chromosomes. When blended, the 23 chromosomes in the oocyte integrate with 23 chromosomes in the spermatozoon and the new configuration of 46 chromosomes is created. This new complete chromosomal profile indicates that the new embryo has been just generated. If the chromosomal profiles’ configuration is wrong — the mosaic embryo with low developmental implantation potency appears. The fertility ‘DevOps’ can screen the IVF–ICSI/IMSI/PICSI generated embryos to exclude the mosaic embryos and the embryos containing the minor or severe abnormalities.
Who can benefit from IVF?
●The couples facing male factor infertility with the low sperm count or low sperm quality.
●The couples facing female factor infertility, especially the Tubal factor when there is an obstruction or abnormality in the Fallopian tubes.
●When the woman is 38 or older.
●When the woman is diagnosed with low ovarian reserve. The ovaries do not produce enough mature eggs during hormone stimulation.
●Long–term sterility.
●Recurrent miscarriages.
●The couple or family has a genetic disease they don’t want to transmit to the child.
How does IVF with own oocytes and sperm work?
As with all treatments, it begins with an initial visit to get information and request the tests. By the second visit, the doctor has already made a diagnosis and the participants sign the consent form.
1. INITIAL IN–PERSON VISIT OR VIDEO–CONFERENCE:
During the first visit, the doctor will ask for information about the couple’s clinical and family history, analyze the situation and run a series of basic diagnostic tests to make a diagnosis and steer the treatment toward the most appropriate assisted reproduction method.
2. REPORT OF MEDICAL TREATMENT TO BE PERFORMED BY THE DOCTOR AND SIGNING OF CONSENT FORMS.
Once all the requested medical tests are performed, the doctor evaluates them and explains the medical treatment required – what procedure will be used, what alternative options there are, the benefits and risks of the process, etc. – and answers any questions the patients might have. Once the entire procedure is understood, the patients sign the consent forms to begin the treatment.
IN VITRO FERTILIZATION WITH THE PATIENT’S OWN EGGS HAS FIVE PHASES:
●Start of ovarian stimulation: involves taking hormones daily to stimulate the ovaries. This phase can last about 15 days and requires going to the clinic three or four times to monitor follicle growth. This is done using an ultrasound and blood test.
●Egg release: once the ovaries are stimulated to produce mature eggs, another hormone is administered to induce ovulation. This process is done in the operating room under sedation, lasts approximately 15 minutes and is painless. Once the patient completely recovers from the sedation, she can go home. The rest is recommended for that day.
●In vitro fertilization process: on the same day that the eggs are retrieved, the semen sample is delivered and processed to isolate the sperm with the highest motility. The most common way to get the semen sample is by masturbation. In those cases where that is not possible, the sperm are extracted directly from the testicle. Once the eggs and sperm are in the laboratory, in vitro fertilization is carried out either conventionally, incubating them jointly, or using a technique called ICSI, which involves injecting the sperm into the egg using a very fine needle.
●Embryo culture and selection: in the days following the in vitro fertilization, biologists will assess the progress of the embryos daily to see which have been properly fertilized and which have not, as well as to check the morphological and morphokinetic development (they are dividing at the right time, not before or after) of the fertilized embryos. To aid in the morphokinetic selection, we use the most advanced laboratory incubation technology, time–lapse incubators, which have a camera inside that allows monitoring the embryos 24 hours a day. This dual selection lets us choose the most viable embryos to implant and yield a healthy baby.
●Selecting the embryos to transfer and embryo transfer. After five days of development, the most viable embryos are selected and transferred to the mother’s uterus.
WHAT ARE MY CHANCES OF GETTING PREGNANT USING MY OWN EGGS?
THE ODDS OF GETTING PREGNANT ARE BETWEEN 40–70%. This depends on multiple factors, including the woman’s age, the quality of the ovarian reserve, the quality of the embryos transferred, and the presence of uterine pathologies or a genetic condition. We can’t provide the odds of overall pregnancy. After studying your case, the medical team will give you a more reliable evaluation of your odds of getting pregnant.
FAQ ABOUT IVF WITH THE MOTHER’S OWN EGGS
IS THE NUMBER OF IVF CYCLES LIMITED OR CAN UNLIMITED TREATMENTS BE ATTEMPTED?
It’s best to do a personalized assessment. Depending on the results and on the progress being made, the doctor will recommend the best course of action for the patient.
WHAT CAN I DO TO IMPROVE MY CHANCES OF GETTING PREGNANT?
At Fertty International we handle everything to ensure your chances of success are as high as possible. We use the techniques needed to optimize the results, such as ICSI or time–lapse, at no extra charge.
HOW MANY EMBRYOS CAN BE TRANSFERRED?
The Assisted Reproduction Law in Spain allows transferring up to three embryos. Transferring more embryos could lead to multiple pregnancies, which is undesired and poses a risk to both the mother and the babies.
WHAT HAPPENS IF A SEMEN SAMPLE CANNOT BE TAKEN ON THE SAME DAY THAT THE EGGS ARE RETRIEVED?
If the male cannot ejaculate on the day of the fertilization, the sperm will be extracted directly from the testicle by microsurgical aspiration. This is a well–tolerated process that is done under local anesthesia and poses no risk.
CAN I CHOOSE OR KNOW WHO THE SEMEN DONOR IS?
We can guarantee the highest compatibility with the female recipient, but you cannot choose the semen donor since they are anonymous by law.
WHAT HAPPENS TO THE EMBRYOS THAT ARE NOT TRANSFERRED?
They can be cryopreserved for future use or donation.